ATP Sulfurylase from Higher Plants
نویسندگان
چکیده
منابع مشابه
ATP sulfurylase from higher plants : purification and preliminary kinetics studies on the cabbage leaf enzyme.
ATP sulfurylase was purified extensively from green cabbage (Brassica capitata L.) leaf. The enzyme appears to be an asymmetric dimer composed of 57,000 dalton subunits. Initial velocity and product inhibition studies of the forward and reverse reactions point to an obligately ordered kinetic mechanism with MgATP adding before MoO(4) (2-) (or SO(4) (2-)). and MgPPi leaving before AMP + MoO(4) (...
متن کاملKinetic mechanism of the dimeric ATP sulfurylase from plants
In plants, sulfur must be obtained from the environment and assimilated into usable forms for metabolism. ATP sulfurylase catalyses the thermodynamically unfavourable formation of a mixed phosphosulfate anhydride in APS (adenosine 5'-phosphosulfate) from ATP and sulfate as the first committed step of sulfur assimilation in plants. In contrast to the multi-functional, allosterically regulated AT...
متن کاملA cDNA clone for an ATP-sulfurylase from Arabidopsis thaliana.
Sulfate is the predominant sulfur source for plants. After uptake from the soil via specific transport proteins into plant roots, sulfate is activated by the enzyme ATP-sulfurylase to APS. ATP-sulfurylase activity in plants is detectable in green tissues and roots (Ellis, 1969; Lunn et al., 1990). Isoforms of the enzyme are localized in plastids and in the cytosol (Lunn et al., 1990; Renosto et...
متن کاملEffect of ATP sulfurylase overexpression in bright yellow 2 tobacco cells. Regulation Of atp sulfurylase and SO4(2-) transport activities.
To determine if the ATP sulfurylase reaction is a regulatory step for the SO4(2-)-assimilation pathway in plants, an Arabidopsis thaliana ATP sulfurylase cDNA, APS2, was fused to the 35S promoter of the cauliflower mosaic virus and introduced by Agrobacterium tumefaciens-mediated transformation into isolated Bright Yellow 2 tobacco (Nicotiana tabacum) cells. The ATP sulfurylase activity in tran...
متن کاملDiversity and regulation of ATP sulfurylase in photosynthetic organisms
ATP sulfurylase (ATPS) catalyzes the first committed step in the sulfate assimilation pathway, the activation of sulfate prior to its reduction. ATPS has been studied in only a few model organisms and even in these cases to a much smaller extent than the sulfate reduction and cysteine synthesis enzymes. This is possibly because the latter were considered of greater regulatory importance for sul...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1982
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.70.1.39